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| 乙酸预处理对相思木酶解糖化及物化性能的影响 |
| Effects of Acetic Acid Pretreatment on Enzymatic Saccharification and Physicochemical Properties of Acacia |
| 收稿日期：2024-12-07 |
| DOI：10.11980/j.issn.0254-508X.2025.05.004 |
| 关键词: 相思木 乙酸预处理 酶解糖化 物化性能 |
| Key Words:Acacia acetic acid pretreatment enzymatic saccharification physicochemical properties |
| 基金项目:广东省普通高校重点科研平台项目（2022KCXTD035）；广州科技职业技术大学2024年度校级科研项目（2024LG17）。 |
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| 摘要:本研究通过对相思木木片进行乙酸预处理、三段磨浆及PFI磨浆，探讨了预处理温度、乙酸用量、PFI打浆转数对相思木酶解糖化率的影响，采用X射线衍射仪、扫描电子显微镜、热重-红外联用仪对预处理前后相思木结构进行分析。结果表明，乙酸预处理可显著提高相思木乙酸提取液中的还原糖得率与固形物含量。在乙酸用量相同（如 HAC5）的情况下，随着预处理温度从140 ℃升高到170 ℃，预处理提取液中的固含量从4.4%升高到16.5%，还原糖得率从2.4%升高到13.2%。在预处理温度为170 ℃时，随着乙酸用量从0增加到9%，预处理液中的固含量从16.1%升高到16.8%，还原糖得率从11.7%升高到13.4%。此外，在一定条件下，预处理温度越高、乙酸用量越大或PFI打浆转数越多，预处理及酶水解转化过程中的总还原糖得率越高，对应原料的质量损失率也越高。综合考虑节约经济成本和环保因素，相思木预处理的最适宜条件为温度170 °C、乙酸体积分数5%、PFI打浆转数5 000转。 |
| Abstract:The effects of pretreatment temperature， acetic acid dosage， and the number of revolutions of PFI refining on the enzymatic hydrolysis saccharification rate of Acacia were investigated through acetic acid pretreatment of wood chips， three-stage refining， and PFI refining in this study. Additionally， XRD， SEM and TG-IR were used to analyze the structure of Acacia before and after pretreatment. The results showed that acetic acid pretreatment could significantly increase the reducing sugar yield and solid content in the acetic acid extract of Acacia. When the acetic acid dosage was the same （e.g. HAC5）， as the pretreatment temperature increased from 140 ℃ to 170 ℃， the solid content in the pretreatment extract increased from 4.4% to 16.5%， and the reducing sugar yield increased from 2.4% to 13.2%. When the pretreatment temperature was 170 ℃， as the acetic acid dosage increased from 0 to 9%， the solid content in the pretreatment solution increased from 16.1% to 16.8%， and the reducing sugar yield increased from 11.7% to 13.4%. In addition， under certain conditions， the higher the pretreatment temperature， the greater the acetic acid dosage， or the more the number of revolutions of PFI refining， the higher the total reducing sugar yield in the pretreatment and enzymatic hydrolysis conversion processes， and the higher the mass loss rate of the corresponding raw materials. Considering the economic cost savings and environmental protection factors comprehensively， the most suitable conditions for the pretreatment of Acacia were the temperature of 170 ℃， the volume fraction of acetic acid of 5%， and 5 000 revolutions of PFI refining. |
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