蒲俊文,姜娜,彭宇康,李文刚,张志刚,齐洪斌,张海涛.CPAM协同纤维素酶预处理提升醋化浆粕乙酰化反应性能的研究[J].中国造纸,2026,45(9):43-51 本文二维码信息
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CPAM协同纤维素酶预处理提升醋化浆粕乙酰化反应性能的研究
Synergistic Effect of CPAM and Cellulase Pretreatment on the Acetylation Reactivity of Acetate-grade Pulp
收稿日期：2026-05-20  修订日期：2026-06-11
DOI：10.11980/j.issn.0254-508X.2026.09.006
关键词:  纤维素酶  醋化浆粕  阳离子聚丙烯酰胺  乙酰化反应  三醋酸纤维素
Key Words:cellulase  acetate-grade pulp  cationic polyacrylamide  acetylation  cellulose triacetate
基金项目:吉林省科技发展计划项目YDZJ202404023QYCX吉林省科技发展计划项目（YDZJ202404023QYCX）。
作者单位邮编
蒲俊文* 1北京林业大学材料科学与技术学院,北京,100083 100083
姜娜 1北京林业大学材料科学与技术学院,北京,100083 100083
彭宇康 1北京林业大学材料科学与技术学院,北京,100083 100083
李文刚 2延边石岘双鹿实业有限责任 公司,吉林图们,133101 133101
张志刚 2延边石岘双鹿实业有限责任 公司,吉林图们,133101 133101
齐洪斌 2延边石岘双鹿实业有限责任 公司,吉林图们,133101 133101
张海涛 2延边石岘双鹿实业有限责任 公司,吉林图们,133101 133101
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摘要:为改善纤维素酶解效果，提升醋化浆粕纤维的乙酰化反应性能，本研究以阳离子聚丙烯酰胺（CPAM）为纤维素酶辅助剂，通过电荷补丁/桥接作用增强酶蛋白在带负电的醋化浆粕纤维上的吸附性能，并经工艺优化确定最佳预处理条件，结合浆粕纤维结构性能变化，探讨了CPAM协同纤维素酶预处理对醋化浆粕乙酰化反应性能的影响。结果表明，CPAM用量250 mg/L时，纤维素酶蛋白吸附量较未添加CPAM提升了60%；在纤维素酶蛋白用量1.0 mg/g、搅拌速度300 r/min条件下，醋化浆粕聚合度降至921。CPAM协同纤维素酶预处理后，醋化浆粕纤维表面粗糙多孔，比表面积、孔体积和保水值分别提升了55.0%、165.2%和24.6%，纤维素结晶区氢键网络结构被有效破坏，纤维素可及度与反应性能显著提升。在满足取代度标准要求下，协同预处理后醋化浆粕制备三醋酸纤维素（CTA）的时间由原醋化浆粕的13 h缩短至8 h，且产物取代度达2.82，CTA产率为91.1%，乙酰化反应性能优于原料和单独纤维素酶处理醋化浆粕。
Abstract:To improve the enzymatic hydrolysis efficiency and enhance the acetylation reactivity of acetate-grade pulp fibers， cationic polyacrylamide (CPAM) was employed as an auxiliary agent for cellulase in this study. CPAM enhanced the adsorption of cellulase proteins onto the negatively charged pulp fibers through charge patching and bridging effects. The optimal pretreatment conditions were determined by process optimization, and the influence of CPAM-assisted cellulase pretreatment on the acetylation reactivity of the pulp was investigated in relation to the changes in fiber structural properties. The results showed that at a CPAM dosage of 250 mg/L, the cellulase protein adsorption increased by 60% compared to that without CPAM. Under the conditions of cellulase protein dosage of 1.0 mg/g and stirring speed of 300 r/min, the polymerization degree of the acetate-grade pulp decreased to 921. After the synergistic pretreatment of CPAM and cellulase, the pulp fiber surface became rough and porous; the specific surface area, pore volume, and water retention value increased by 55.0%, 165.2%, and 24.6%, respectively. The hydrogen-bond network in the crystalline region of cellulose was effectively disrupted, and the accessibility and reactivity of cellulose were notably improved. While meeting the required degree of substitution standard, the time for preparing cellulose triacetate (CTA) from the synergistically pretreated acetate-grade pulp was shortened from 13 h (for the original pulp) to 8 h. The resulting CTA had a degree of substitution of 2.82 and a yield of 91.1%. It demonstrated the acetylation performance of the synergistically pretreated acetate-grade pulp was superior to that of both the original pulp and the pulp treated with cellulase alone.
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