| 裴继诚,于秀玲,张方东.漆酶/对羟基苯甲酸丁酯处理未漂浆提高抗菌性能的研究[J].中国造纸,2011,30(9):11-14 |
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| 漆酶/对羟基苯甲酸丁酯处理未漂浆提高抗菌性能的研究 |
| Improving the Antimicrobial Activity of Unbleached Kraft Pulp by Treatment with Laccase and Butyl 4-Hydroxybenzoate |
| 收稿日期： |
| DOI：10.11980/j.issn.0254-508X.2011.09.003 |
| 关键词: 漆酶 对羟基苯甲酸丁酯 未漂硫酸盐浆 抗菌活性 |
| Key Words:lacccase butyl 4-hydroxybenzoate unbleached kraft pulp antimicrobial activity |
| 基金项目: |
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| 摘要:从提高纸张抗菌性能角度出发，研究了漆酶/对羟基苯甲酸丁酯处理未漂硫酸盐浆后纸样抗菌性能的变化。结果表明，漆酶/对羟基苯甲酸丁酯处理未漂硫酸盐浆抄造的纸样能较好地抑制细菌的生长，对金黄色葡萄球菌、枯草芽孢杆菌、大肠杆菌和沙门氏菌的抑菌率分别为94.50%、90.87%、98.99%和99.12%。耐洗实验结果表明，纸样经4次洗脱后其抑菌率变化不大，推测对羟基苯甲酸丁酯在漆酶作用下与纤维发生化学键连接而非简单的物理吸附。通过浆料卡伯值的测定和扫描电镜的检测结果间接表明了对羟基苯甲酸丁酯在漆酶的作用下可以接枝到纤维表面，表现为卡伯值增加，纤维表面有沉积物及纤维间生成连接膜。 |
| Abstract:In order to improve the antimicrobial activity of paper, the antimicrobial activity of the paper manufactured with the kraft pulp which was treated with laccase/buty1 4-hydroxybenzoate was evaluated. The experimental results showed that, the handsheet paper containing laccase and butyl 4-hydroxybenzoate showed a greater efficiency against the growth of bacteria, which resisted s.aureus, bacillus subtilis, e.coli, salmonella to 94.50%, 90.87%, 98.99%, and 99.12% respectively. There was little change of anti-bacteria effect of the handsheet paper after washing four times, which indicated that the chemical bonds between butyl 4-hydroxybenzoate and fibers probably were formed in the presence of laccase. After the enzymatic treatment, kappa number increase compared with the control sample, and the observation of scanning electron microscope(SEM) indicated that fibers treated by laccase and butyl 4-hydroxybenzoate were agglutinated together in wide area, which indicated that the phenol compounds may be linked to the surface of fibers by covalent bond to some extent. |
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